|
KMID : 0525720150200040280
|
|
Journal of Chitin and Chitosan
2015 Volume.20 No. 4 p.280 ~ p.286
|
|
|
Active Molecular Chitosan (AMC-S1): Preparation and Characterization of Antibacterial Activity
|
|
Kim Joo-Won
Lee Choon-Geun
Hwang You-Jin
Park Jae-Kweon
|
|
|
Abstract
|
|
|
|
The aim of the present study is to prepare the chitosan hydrolysates and characterize their potential antibacterial activity. High molecular weight chitosan (CTSN-H) and medium molecular weight chitosan (MMWC, 2~10-kDa) were further degraded efficiently by using chitosanase from Bacillus subtilis JH-1122. Molecular weight (MW) of the final product was determined by MALDI-TOF MS, showed several peaks ranging from 0.8 to 2.5-kDa. Based on antibacterial activity of the hydrolysates, final products were named as active molecular chitosan (AMC-S1). AMC-S1 was evaluated as IC50 to 12.5 μg/mL of the concentration toward Candida albicans. Our present study demonstrates that AMC-S1 derived from the digestion of CTSN-H and MMWC can be selected as a potent factor affecting on antimicrobial activity against C. albicans for the industry and pharmaceutical applications.
|
|
|
KEYWORD
|
|
|
Active molecular chitosan, AMC-S1, Chitosanase, MALDI-TOF MS, Candida albicans, antibacterial activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|